Construct: shRNA TRCN0000192505

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_025476.3-495s1c1
DNA Barcode:
GCAATCTGCATTAGTGATGTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Rmdn1 (66302)
Gene Description:
regulator of microtubule dynamics 1
Transcript:
RefSeq NM_025476.3 (NON-CURRENT)
Match location:
Position 495 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000192505)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAATCTGCATTAGTGATGTT
Hairpin Sequence:
5'-CCGG-GCAATCTGCATTAGTGATGTT-CTCGAG-AACATCACTAATGCAGATTGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAATCTGCATTAGTGATGTTCTCGAGAACATCACTAATGCAGATTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAATCTGCATTAGTGATGTTCTCGAGAACATCACTAATGCAGATTGC-3'

Other clones with same target sequence:

  1. TRCN0000319877