Construct: shRNA TRCN0000192528

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_177238.2-2383s1c1
DNA Barcode:
GCTTTGCAACTGCAAACTTTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
9830147P19Rik (320711)
Gene Description:
RIKEN cDNA 9830147P19 gene
Transcript:
RefSeq NM_177238.2 (NON-CURRENT)
Match location:
Position 2383 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000192528)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTTTGCAACTGCAAACTTTA
Hairpin Sequence:
5'-CCGG-GCTTTGCAACTGCAAACTTTA-CTCGAG-TAAAGTTTGCAGTTGCAAAGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTTTGCAACTGCAAACTTTACTCGAGTAAAGTTTGCAGTTGCAAAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTTTGCAACTGCAAACTTTACTCGAGTAAAGTTTGCAGTTGCAAAGC-3'

Other clones with same target sequence:

(none)