Construct: shRNA TRCN0000192665

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_177266.3-948s1c1
DNA Barcode:
GCAGACTTAGATGATGAGTTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gfm2 (320806)
Gene Description:
G elongation factor, mitochondrial 2
Transcript:
RefSeq NM_177266.3 (NON-CURRENT)
Match location:
Position 948 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000192665)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGACTTAGATGATGAGTTT
Hairpin Sequence:
5'-CCGG-GCAGACTTAGATGATGAGTTT-CTCGAG-AAACTCATCATCTAAGTCTGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGACTTAGATGATGAGTTTCTCGAGAAACTCATCATCTAAGTCTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGACTTAGATGATGAGTTTCTCGAGAAACTCATCATCTAAGTCTGC-3'

Other clones with same target sequence:

(none)