Construct: shRNA TRCN0000192682

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_178764.2-568s1c1
DNA Barcode:
GTATCCAATCAGAAGTGCCTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Fam168a (319604)
Gene Description:
family with sequence similarity 168, member A
Transcript:
RefSeq NM_178764.2 (NON-CURRENT)
Match location:
Position 568 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000192682)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTATCCAATCAGAAGTGCCTA
Hairpin Sequence:
5'-CCGG-GTATCCAATCAGAAGTGCCTA-CTCGAG-TAGGCACTTCTGATTGGATAC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTATCCAATCAGAAGTGCCTACTCGAGTAGGCACTTCTGATTGGATACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTATCCAATCAGAAGTGCCTACTCGAGTAGGCACTTCTGATTGGATAC-3'

Other clones with same target sequence:

(none)