Construct: shRNA TRCN0000192688

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_015764.1-5740s1c1
DNA Barcode:
GCTGGAGAAATTCCTCCAATA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Greb1 (268527)
Gene Description:
gene regulated by estrogen in breast cancer protein
Transcript:
RefSeq NM_015764.1 (NON-CURRENT)
Match location:
Position 5740 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000192688)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGGAGAAATTCCTCCAATA
Hairpin Sequence:
5'-CCGG-GCTGGAGAAATTCCTCCAATA-CTCGAG-TATTGGAGGAATTTCTCCAGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGGAGAAATTCCTCCAATACTCGAGTATTGGAGGAATTTCTCCAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGGAGAAATTCCTCCAATACTCGAGTATTGGAGGAATTTCTCCAGC-3'

Other clones with same target sequence:

(none)