Construct: shRNA TRCN0000192748

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_178750.4-3585s1c1
DNA Barcode:
GCTGTGTTAGTGTGTGTTAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ss18l1 (269397)
Gene Description:
SS18, nBAF chromatin remodeling complex subunit like 1
Transcript:
RefSeq NM_178750.4 (NON-CURRENT)
Match location:
Position 3585 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000192748)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGTGTTAGTGTGTGTTAAT
Hairpin Sequence:
5'-CCGG-GCTGTGTTAGTGTGTGTTAAT-CTCGAG-ATTAACACACACTAACACAGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGTGTTAGTGTGTGTTAATCTCGAGATTAACACACACTAACACAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGTGTTAGTGTGTGTTAATCTCGAGATTAACACACACTAACACAGC-3'

Other clones with same target sequence:

(none)