Construct: shRNA TRCN0000192750

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_183217.1-539s1c1
DNA Barcode:
GCTATTTATTGCCAGTGAGCA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Dennd1b (329260)
Gene Description:
DENN/MADD domain containing 1B
Transcript:
RefSeq NM_183217.1 (NON-CURRENT)
Match location:
Position 539 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000192750)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTATTTATTGCCAGTGAGCA
Hairpin Sequence:
5'-CCGG-GCTATTTATTGCCAGTGAGCA-CTCGAG-TGCTCACTGGCAATAAATAGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTATTTATTGCCAGTGAGCACTCGAGTGCTCACTGGCAATAAATAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTATTTATTGCCAGTGAGCACTCGAGTGCTCACTGGCAATAAATAGC-3'

Other clones with same target sequence:

(none)