Construct: shRNA TRCN0000192802

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_144920.2-1844s1c1
DNA Barcode:
GCCTTTCTCATCAGTTAAGTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Plekha5 (109135)
Gene Description:
pleckstrin homology domain containing, family A member 5
Transcript:
RefSeq NM_144920.2 (NON-CURRENT)
Match location:
Position 1844 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000192802)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCTTTCTCATCAGTTAAGTA
Hairpin Sequence:
5'-CCGG-GCCTTTCTCATCAGTTAAGTA-CTCGAG-TACTTAACTGATGAGAAAGGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCTTTCTCATCAGTTAAGTACTCGAGTACTTAACTGATGAGAAAGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCTTTCTCATCAGTTAAGTACTCGAGTACTTAACTGATGAGAAAGGC-3'

Other clones with same target sequence:

  1. TRCN0000329298