Construct: shRNA TRCN0000192935

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001013024.1-2324s1c1
DNA Barcode:
GCAACGAATCATAACCTGGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Usp13 (72607)
Gene Description:
ubiquitin specific peptidase 13 (isopeptidase T-3)
Transcript:
RefSeq NM_001013024.1 (NON-CURRENT)
Match location:
Position 2324 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000192935)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAACGAATCATAACCTGGAA
Hairpin Sequence:
5'-CCGG-GCAACGAATCATAACCTGGAA-CTCGAG-TTCCAGGTTATGATTCGTTGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAACGAATCATAACCTGGAACTCGAGTTCCAGGTTATGATTCGTTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAACGAATCATAACCTGGAACTCGAGTTCCAGGTTATGATTCGTTGC-3'

Other clones with same target sequence:

(none)