Construct: shRNA TRCN0000192959

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001005247.1-4396s1c1
DNA Barcode:
GCCTCCTGAATGTTAGAATTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Hps5 (246694)
Gene Description:
HPS5, biogenesis of lysosomal organelles complex 2 subunit 2
Transcript:
RefSeq NM_001005247.1 (NON-CURRENT)
Match location:
Position 4396 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000192959)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCTCCTGAATGTTAGAATTA
Hairpin Sequence:
5'-CCGG-GCCTCCTGAATGTTAGAATTA-CTCGAG-TAATTCTAACATTCAGGAGGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCTCCTGAATGTTAGAATTACTCGAGTAATTCTAACATTCAGGAGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCTCCTGAATGTTAGAATTACTCGAGTAATTCTAACATTCAGGAGGC-3'

Other clones with same target sequence:

(none)