Construct: shRNA TRCN0000193063

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_173760.3-3409s1c1
DNA Barcode:
CCGAAAGTTTCCATGCTTATA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ppip5k2 (227399)
Gene Description:
diphosphoinositol pentakisphosphate kinase 2
Transcript:
RefSeq NM_173760.3 (NON-CURRENT)
Match location:
Position 3409 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000193063)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCGAAAGTTTCCATGCTTATA
Hairpin Sequence:
5'-CCGG-CCGAAAGTTTCCATGCTTATA-CTCGAG-TATAAGCATGGAAACTTTCGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCGAAAGTTTCCATGCTTATACTCGAGTATAAGCATGGAAACTTTCGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCGAAAGTTTCCATGCTTATACTCGAGTATAAGCATGGAAACTTTCGG-3'

Other clones with same target sequence:

(none)