Construct: shRNA TRCN0000193075

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_019465.1-977s1c2
DNA Barcode:
CTCTAGAAAGTTACAGATCAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Crtam (54698)
Gene Description:
cytotoxic and regulatory T cell molecule
Transcript:
RefSeq NM_019465.1 (NON-CURRENT)
Match location:
Position 977 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000193075)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTCTAGAAAGTTACAGATCAA
Hairpin Sequence:
5'-CCGG-CTCTAGAAAGTTACAGATCAA-CTCGAG-TTGATCTGTAACTTTCTAGAG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTCTAGAAAGTTACAGATCAACTCGAGTTGATCTGTAACTTTCTAGAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTCTAGAAAGTTACAGATCAACTCGAGTTGATCTGTAACTTTCTAGAG-3'

Other clones with same target sequence:

(none)