Construct: shRNA TRCN0000193089

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_177354.2-1734s1c2
DNA Barcode:
CAGGGACACAATTCTTTGAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Vash1 (238328)
Gene Description:
vasohibin 1
Transcript:
RefSeq NM_177354.2 (NON-CURRENT)
Match location:
Position 1734 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000193089)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAGGGACACAATTCTTTGAAA
Hairpin Sequence:
5'-CCGG-CAGGGACACAATTCTTTGAAA-CTCGAG-TTTCAAAGAATTGTGTCCCTG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAGGGACACAATTCTTTGAAACTCGAGTTTCAAAGAATTGTGTCCCTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAGGGACACAATTCTTTGAAACTCGAGTTTCAAAGAATTGTGTCCCTG-3'

Other clones with same target sequence:

(none)