Construct: shRNA TRCN0000193100

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_027968.1-441s1c2
DNA Barcode:
CGTTAGTTATTCAGACCGGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Fbxo30 (71865)
Gene Description:
F-box protein 30
Transcript:
RefSeq NM_027968.1 (NON-CURRENT)
Match location:
Position 441 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000193100)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGTTAGTTATTCAGACCGGAA
Hairpin Sequence:
5'-CCGG-CGTTAGTTATTCAGACCGGAA-CTCGAG-TTCCGGTCTGAATAACTAACG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGTTAGTTATTCAGACCGGAACTCGAGTTCCGGTCTGAATAACTAACGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGTTAGTTATTCAGACCGGAACTCGAGTTCCGGTCTGAATAACTAACG-3'

Other clones with same target sequence:

(none)