Construct: shRNA TRCN0000193142

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_013632.2-108s1c2
DNA Barcode:
CTTCTGCAACACACTGAATAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Pnp (18950)
Gene Description:
purine-nucleoside phosphorylase
Transcript:
RefSeq NM_013632.2 (NON-CURRENT)
Match location:
Position 108 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000193142)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTTCTGCAACACACTGAATAT
Hairpin Sequence:
5'-CCGG-CTTCTGCAACACACTGAATAT-CTCGAG-ATATTCAGTGTGTTGCAGAAG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTTCTGCAACACACTGAATATCTCGAGATATTCAGTGTGTTGCAGAAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTTCTGCAACACACTGAATATCTCGAGATATTCAGTGTGTTGCAGAAG-3'

Other clones with same target sequence:

(none)