Construct: shRNA TRCN0000193227

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_181411.2-1172s1c1
DNA Barcode:
CAACAGGATGAGTTTCTAAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Aftph (216549)
Gene Description:
aftiphilin
Transcript:
RefSeq NM_181411.2 (NON-CURRENT)
Match location:
Position 1172 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000193227)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAACAGGATGAGTTTCTAAAT
Hairpin Sequence:
5'-CCGG-CAACAGGATGAGTTTCTAAAT-CTCGAG-ATTTAGAAACTCATCCTGTTG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAACAGGATGAGTTTCTAAATCTCGAGATTTAGAAACTCATCCTGTTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAACAGGATGAGTTTCTAAATCTCGAGATTTAGAAACTCATCCTGTTG-3'

Other clones with same target sequence:

  1. TRCN0000277411