Construct: shRNA TRCN0000193233

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_172407.1-1571s1c1
DNA Barcode:
CAAGAAAGTATTGTCTGTGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Cdkn2aip (70925)
Gene Description:
CDKN2A interacting protein
Transcript:
RefSeq NM_172407.1 (NON-CURRENT)
Match location:
Position 1571 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000193233)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAAGAAAGTATTGTCTGTGAA
Hairpin Sequence:
5'-CCGG-CAAGAAAGTATTGTCTGTGAA-CTCGAG-TTCACAGACAATACTTTCTTG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAAGAAAGTATTGTCTGTGAACTCGAGTTCACAGACAATACTTTCTTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAAGAAAGTATTGTCTGTGAACTCGAGTTCACAGACAATACTTTCTTG-3'

Other clones with same target sequence:

(none)