Construct: shRNA TRCN0000193241

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_201360.1-87s1c1
DNA Barcode:
CCATATTCACAGTCATCTTCA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Cyp2d12 (380997)
Gene Description:
cytochrome P450, family 2, subfamily d, polypeptide 12
Transcript:
RefSeq NM_201360.1 (CURRENT)
Match location:
Position 87 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000193241)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCATATTCACAGTCATCTTCA
Hairpin Sequence:
5'-CCGG-CCATATTCACAGTCATCTTCA-CTCGAG-TGAAGATGACTGTGAATATGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCATATTCACAGTCATCTTCACTCGAGTGAAGATGACTGTGAATATGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCATATTCACAGTCATCTTCACTCGAGTGAAGATGACTGTGAATATGG-3'

Other clones with same target sequence:

(none)