Construct: shRNA TRCN0000193306

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_033218.1-2303s1c1
DNA Barcode:
CTTGGCTAGCTACTTCTTAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Srebf2 (20788)
Gene Description:
sterol regulatory element binding factor 2
Transcript:
RefSeq NM_033218.1 (CURRENT)
Match location:
Position 2303 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000193306)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTTGGCTAGCTACTTCTTAAA
Hairpin Sequence:
5'-CCGG-CTTGGCTAGCTACTTCTTAAA-CTCGAG-TTTAAGAAGTAGCTAGCCAAG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTTGGCTAGCTACTTCTTAAACTCGAGTTTAAGAAGTAGCTAGCCAAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTTGGCTAGCTACTTCTTAAACTCGAGTTTAAGAAGTAGCTAGCCAAG-3'

Other clones with same target sequence:

(none)