Construct: shRNA TRCN0000193413

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_178608.2-2667s1c1
DNA Barcode:
CCCGAAATAATAAATGGCTTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Reep1 (52250)
Gene Description:
receptor accessory protein 1
Transcript:
RefSeq NM_178608.2 (NON-CURRENT)
Match location:
Position 2667 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000193413)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCGAAATAATAAATGGCTTT
Hairpin Sequence:
5'-CCGG-CCCGAAATAATAAATGGCTTT-CTCGAG-AAAGCCATTTATTATTTCGGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCGAAATAATAAATGGCTTTCTCGAGAAAGCCATTTATTATTTCGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCGAAATAATAAATGGCTTTCTCGAGAAAGCCATTTATTATTTCGGG-3'

Other clones with same target sequence:

  1. TRCN0000292969