Construct: shRNA TRCN0000193432

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_009767.1-399s1c1
DNA Barcode:
GAAGAATTTAAGACCAGCATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Chic1 (12212)
Gene Description:
cysteine-rich hydrophobic domain 1
Transcript:
RefSeq NM_009767.1 (NON-CURRENT)
Match location:
Position 399 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000193432)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAAGAATTTAAGACCAGCATT
Hairpin Sequence:
5'-CCGG-GAAGAATTTAAGACCAGCATT-CTCGAG-AATGCTGGTCTTAAATTCTTC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAAGAATTTAAGACCAGCATTCTCGAGAATGCTGGTCTTAAATTCTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAAGAATTTAAGACCAGCATTCTCGAGAATGCTGGTCTTAAATTCTTC-3'

Other clones with same target sequence:

(none)