Construct: shRNA TRCN0000193479

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_029478.2-1652s1c1
DNA Barcode:
GCTTGTCACAAATGATTGTTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Vmp1 (75909)
Gene Description:
vacuole membrane protein 1
Transcript:
RefSeq NM_029478.2 (NON-CURRENT)
Match location:
Position 1652 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000193479)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTTGTCACAAATGATTGTTT
Hairpin Sequence:
5'-CCGG-GCTTGTCACAAATGATTGTTT-CTCGAG-AAACAATCATTTGTGACAAGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTTGTCACAAATGATTGTTTCTCGAGAAACAATCATTTGTGACAAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTTGTCACAAATGATTGTTTCTCGAGAAACAATCATTTGTGACAAGC-3'

Other clones with same target sequence:

(none)