Construct: shRNA TRCN0000193492

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_145920.2-3802s1c1
DNA Barcode:
GCTTGCAGTATACATATGCTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Evc2 (68525)
Gene Description:
EvC ciliary complex subunit 2
Transcript:
RefSeq NM_145920.2 (NON-CURRENT)
Match location:
Position 3802 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000193492)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTTGCAGTATACATATGCTT
Hairpin Sequence:
5'-CCGG-GCTTGCAGTATACATATGCTT-CTCGAG-AAGCATATGTATACTGCAAGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTTGCAGTATACATATGCTTCTCGAGAAGCATATGTATACTGCAAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTTGCAGTATACATATGCTTCTCGAGAAGCATATGTATACTGCAAGC-3'

Other clones with same target sequence:

(none)