Construct: shRNA TRCN0000193508

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001033158.1-689s1c1
DNA Barcode:
GTACTTTCAACACTCTCTCTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Rasl12 (70784)
Gene Description:
RAS-like, family 12
Transcript:
RefSeq NM_001033158.1 (NON-CURRENT)
Match location:
Position 689 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000193508)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTACTTTCAACACTCTCTCTA
Hairpin Sequence:
5'-CCGG-GTACTTTCAACACTCTCTCTA-CTCGAG-TAGAGAGAGTGTTGAAAGTAC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTACTTTCAACACTCTCTCTACTCGAGTAGAGAGAGTGTTGAAAGTACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTACTTTCAACACTCTCTCTACTCGAGTAGAGAGAGTGTTGAAAGTAC-3'

Other clones with same target sequence:

(none)