Construct: shRNA TRCN0000193537

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001033633.1-5066s1c1
DNA Barcode:
GCACAGAATTTATGGATCTTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Slc2a13 (239606)
Gene Description:
solute carrier family 2 (facilitated glucose transporter), member 13
Transcript:
RefSeq NM_001033633.1 (NON-CURRENT)
Match location:
Position 5066 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000193537)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCACAGAATTTATGGATCTTT
Hairpin Sequence:
5'-CCGG-GCACAGAATTTATGGATCTTT-CTCGAG-AAAGATCCATAAATTCTGTGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCACAGAATTTATGGATCTTTCTCGAGAAAGATCCATAAATTCTGTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCACAGAATTTATGGATCTTTCTCGAGAAAGATCCATAAATTCTGTGC-3'

Other clones with same target sequence:

(none)