Construct: shRNA TRCN0000193552

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001024911.1-1410s1c1
DNA Barcode:
GCCTTATCAGTAACTCAGTTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Sept10 (103080)
Gene Description:
septin 10
Transcript:
RefSeq NM_001024911.1 (NON-CURRENT)
Match location:
Position 1410 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000193552)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCTTATCAGTAACTCAGTTA
Hairpin Sequence:
5'-CCGG-GCCTTATCAGTAACTCAGTTA-CTCGAG-TAACTGAGTTACTGATAAGGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCTTATCAGTAACTCAGTTACTCGAGTAACTGAGTTACTGATAAGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCTTATCAGTAACTCAGTTACTCGAGTAACTGAGTTACTGATAAGGC-3'

Other clones with same target sequence:

  1. TRCN0000347588