Construct: shRNA TRCN0000193671

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_008717.1-4893s1c2
DNA Barcode:
CCAAATCGAAAGAGGAACCAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Zfp638 (18139)
Gene Description:
zinc finger protein 638
Transcript:
RefSeq NM_008717.1 (NON-CURRENT)
Match location:
Position 4893 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000193671)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAAATCGAAAGAGGAACCAT
Hairpin Sequence:
5'-CCGG-CCAAATCGAAAGAGGAACCAT-CTCGAG-ATGGTTCCTCTTTCGATTTGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAAATCGAAAGAGGAACCATCTCGAGATGGTTCCTCTTTCGATTTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAAATCGAAAGAGGAACCATCTCGAGATGGTTCCTCTTTCGATTTGG-3'

Other clones with same target sequence:

(none)