Construct: shRNA TRCN0000193703

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_010008.2-537s1c1
DNA Barcode:
CCTCTAAGTGTTATGCAAGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Cyp2j6 (13110)
Gene Description:
cytochrome P450, family 2, subfamily j, polypeptide 6
Transcript:
RefSeq NM_010008.2 (NON-CURRENT)
Match location:
Position 537 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000193703)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTCTAAGTGTTATGCAAGAA
Hairpin Sequence:
5'-CCGG-CCTCTAAGTGTTATGCAAGAA-CTCGAG-TTCTTGCATAACACTTAGAGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTCTAAGTGTTATGCAAGAACTCGAGTTCTTGCATAACACTTAGAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTCTAAGTGTTATGCAAGAACTCGAGTTCTTGCATAACACTTAGAGG-3'

Other clones with same target sequence:

(none)