Construct: shRNA TRCN0000193756

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_029935.2-3263s1c1
DNA Barcode:
CCCTTAGAATGTCCTCAGAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Chst15 (77590)
Gene Description:
carbohydrate (N-acetylgalactosamine 4-sulfate 6-O) sulfotransferase 15
Transcript:
RefSeq NM_029935.2 (NON-CURRENT)
Match location:
Position 3263 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000193756)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCTTAGAATGTCCTCAGAAA
Hairpin Sequence:
5'-CCGG-CCCTTAGAATGTCCTCAGAAA-CTCGAG-TTTCTGAGGACATTCTAAGGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCTTAGAATGTCCTCAGAAACTCGAGTTTCTGAGGACATTCTAAGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCTTAGAATGTCCTCAGAAACTCGAGTTTCTGAGGACATTCTAAGGG-3'

Other clones with same target sequence:

(none)