Construct: shRNA TRCN0000193756
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_029935.2-3263s1c1
- DNA Barcode:
- CCCTTAGAATGTCCTCAGAAA
Original Target:
- Taxon:
-
Mus musculus (mouse)
- Gene:
- Chst15 (77590)
- Gene Description:
- carbohydrate (N-acetylgalactosamine 4-sulfate 6-O) sulfotransferase 15
- Transcript:
- RefSeq NM_029935.2 (NON-CURRENT)
- Match location:
- Position 3263 (3UTR)
Vector Information:
- Vector Backbone:
- pLKO.1
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000193756)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- CCCTTAGAATGTCCTCAGAAA
- Hairpin Sequence:
-
5'-CCGG-CCCTTAGAATGTCCTCAGAAA-CTCGAG-TTTCTGAGGACATTCTAAGGG-TTTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGCCCTTAGAATGTCCTCAGAAACTCGAGTTTCTGAGGACATTCTAAGGGTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAACCCTTAGAATGTCCTCAGAAACTCGAGTTTCTGAGGACATTCTAAGGG-3'
Other clones with same target sequence:
(none)
