Construct: shRNA TRCN0000193799

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001025577.2-1054s1c1
DNA Barcode:
CAAGGAGAAATACGAGAAGCT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Maf (17132)
Gene Description:
avian musculoaponeurotic fibrosarcoma oncogene homolog
Transcript:
RefSeq NM_001025577.2 (CURRENT)
Match location:
Position 1054 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000193799)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAAGGAGAAATACGAGAAGCT
Hairpin Sequence:
5'-CCGG-CAAGGAGAAATACGAGAAGCT-CTCGAG-AGCTTCTCGTATTTCTCCTTG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAAGGAGAAATACGAGAAGCTCTCGAGAGCTTCTCGTATTTCTCCTTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAAGGAGAAATACGAGAAGCTCTCGAGAGCTTCTCGTATTTCTCCTTG-3'

Other clones with same target sequence:

  1. TRCN0000423501