Construct: shRNA TRCN0000193841

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_153085.1-436s1c1
DNA Barcode:
CCAGTTACTCTCCACAAGAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Wac (225131)
Gene Description:
WW domain containing adaptor with coiled-coil
Transcript:
RefSeq NM_153085.1 (NON-CURRENT)
Match location:
Position 436 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000193841)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGTTACTCTCCACAAGAAA
Hairpin Sequence:
5'-CCGG-CCAGTTACTCTCCACAAGAAA-CTCGAG-TTTCTTGTGGAGAGTAACTGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGTTACTCTCCACAAGAAACTCGAGTTTCTTGTGGAGAGTAACTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGTTACTCTCCACAAGAAACTCGAGTTTCTTGTGGAGAGTAACTGG-3'

Other clones with same target sequence:

  1. TRCN0000345718