Construct: shRNA TRCN0000193865

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001013792.1-387s1c1
DNA Barcode:
CCTGATGTTAAACAGAGCCAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Otulin (432940)
Gene Description:
OTU deubiquitinase with linear linkage specificity
Transcript:
RefSeq NM_001013792.1 (NON-CURRENT)
Match location:
Position 387 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000193865)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGATGTTAAACAGAGCCAT
Hairpin Sequence:
5'-CCGG-CCTGATGTTAAACAGAGCCAT-CTCGAG-ATGGCTCTGTTTAACATCAGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGATGTTAAACAGAGCCATCTCGAGATGGCTCTGTTTAACATCAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGATGTTAAACAGAGCCATCTCGAGATGGCTCTGTTTAACATCAGG-3'

Other clones with same target sequence:

  1. TRCN0000347227