Construct: shRNA TRCN0000193909

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_177618.4-1766s1c1
DNA Barcode:
CCGGAGTTTGTGAATAGCTAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Wscd1 (216881)
Gene Description:
WSC domain containing 1
Transcript:
RefSeq NM_177618.4 (CURRENT)
Match location:
Position 1766 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000193909)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCGGAGTTTGTGAATAGCTAT
Hairpin Sequence:
5'-CCGG-CCGGAGTTTGTGAATAGCTAT-CTCGAG-ATAGCTATTCACAAACTCCGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCGGAGTTTGTGAATAGCTATCTCGAGATAGCTATTCACAAACTCCGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCGGAGTTTGTGAATAGCTATCTCGAGATAGCTATTCACAAACTCCGG-3'

Other clones with same target sequence:

(none)