Construct: shRNA TRCN0000193946

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001008421.1-290s1c1
DNA Barcode:
GTCCGGTGTTATGACACATAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Nol10 (217431)
Gene Description:
nucleolar protein 10
Transcript:
RefSeq NM_001008421.1 (CURRENT)
Match location:
Position 290 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000193946)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTCCGGTGTTATGACACATAT
Hairpin Sequence:
5'-CCGG-GTCCGGTGTTATGACACATAT-CTCGAG-ATATGTGTCATAACACCGGAC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTCCGGTGTTATGACACATATCTCGAGATATGTGTCATAACACCGGACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTCCGGTGTTATGACACATATCTCGAGATATGTGTCATAACACCGGAC-3'

Other clones with same target sequence:

(none)