Construct: shRNA TRCN0000194044

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_134175.1-196s1c1
DNA Barcode:
GAGTCACTGAACATTGAGAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Vmn1r6 (171193)
Gene Description:
vomeronasal 1 receptor 6
Transcript:
RefSeq NM_134175.1 (CURRENT)
Match location:
Position 196 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000194044)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGTCACTGAACATTGAGAAT
Hairpin Sequence:
5'-CCGG-GAGTCACTGAACATTGAGAAT-CTCGAG-ATTCTCAATGTTCAGTGACTC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGTCACTGAACATTGAGAATCTCGAGATTCTCAATGTTCAGTGACTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGTCACTGAACATTGAGAATCTCGAGATTCTCAATGTTCAGTGACTC-3'

Other clones with same target sequence:

(none)