Construct: shRNA TRCN0000194115

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_177328.2-931s1c1
DNA Barcode:
GTATCTACTCTTGCATCTGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Grm7 (108073)
Gene Description:
glutamate receptor, metabotropic 7
Transcript:
RefSeq NM_177328.2 (NON-CURRENT)
Match location:
Position 931 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000194115)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTATCTACTCTTGCATCTGAA
Hairpin Sequence:
5'-CCGG-GTATCTACTCTTGCATCTGAA-CTCGAG-TTCAGATGCAAGAGTAGATAC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTATCTACTCTTGCATCTGAACTCGAGTTCAGATGCAAGAGTAGATACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTATCTACTCTTGCATCTGAACTCGAGTTCAGATGCAAGAGTAGATAC-3'

Other clones with same target sequence:

(none)