Construct: shRNA TRCN0000194159

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_024124.2-932s1c1
DNA Barcode:
GCTCAAGATAGCAAGGATGAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Hdac9 (79221)
Gene Description:
histone deacetylase 9
Transcript:
RefSeq NM_024124.2 (NON-CURRENT)
Match location:
Position 932 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000194159)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTCAAGATAGCAAGGATGAT
Hairpin Sequence:
5'-CCGG-GCTCAAGATAGCAAGGATGAT-CTCGAG-ATCATCCTTGCTATCTTGAGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTCAAGATAGCAAGGATGATCTCGAGATCATCCTTGCTATCTTGAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTCAAGATAGCAAGGATGATCTCGAGATCATCCTTGCTATCTTGAGC-3'

Other clones with same target sequence:

(none)