Construct: shRNA TRCN0000194209

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_018887.2-2304s1c1
DNA Barcode:
GCAGTCTCATTATGTCTGGTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Cyp39a1 (56050)
Gene Description:
cytochrome P450, family 39, subfamily a, polypeptide 1
Transcript:
RefSeq NM_018887.2 (NON-CURRENT)
Match location:
Position 2304 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000194209)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGTCTCATTATGTCTGGTT
Hairpin Sequence:
5'-CCGG-GCAGTCTCATTATGTCTGGTT-CTCGAG-AACCAGACATAATGAGACTGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGTCTCATTATGTCTGGTTCTCGAGAACCAGACATAATGAGACTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGTCTCATTATGTCTGGTTCTCGAGAACCAGACATAATGAGACTGC-3'

Other clones with same target sequence:

(none)