Construct: shRNA TRCN0000194269

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_357498.1-225s1c2
DNA Barcode:
CCTGCTCATCTCTCTCATCTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Vmn2r16 (384220)
Gene Description:
vomeronasal 2, receptor 16
Transcript:
RefSeq XM_357498.1 (NON-CURRENT)
Match location:
Position 225 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000194269)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGCTCATCTCTCTCATCTT
Hairpin Sequence:
5'-CCGG-CCTGCTCATCTCTCTCATCTT-CTCGAG-AAGATGAGAGAGATGAGCAGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGCTCATCTCTCTCATCTTCTCGAGAAGATGAGAGAGATGAGCAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGCTCATCTCTCTCATCTTCTCGAGAAGATGAGAGAGATGAGCAGG-3'

Other clones with same target sequence:

(none)