Construct: shRNA TRCN0000194286

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006873.2-1898s1c2
DNA Barcode:
GCAGTGGTATGGAAGATAGAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
STON1 (11037)
Gene Description:
stonin 1
Transcript:
RefSeq NM_006873.2 (NON-CURRENT)
Match location:
Position 1898 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000194286)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGTGGTATGGAAGATAGAT
Hairpin Sequence:
5'-CCGG-GCAGTGGTATGGAAGATAGAT-CTCGAG-ATCTATCTTCCATACCACTGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGTGGTATGGAAGATAGATCTCGAGATCTATCTTCCATACCACTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGTGGTATGGAAGATAGATCTCGAGATCTATCTTCCATACCACTGC-3'

Other clones with same target sequence:

(none)