Construct: shRNA TRCN0000194310

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_372500.1-655s1c2
DNA Barcode:
GCAGTCATTCTTTGTCGCATA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
OR4N2 (390429)
Gene Description:
olfactory receptor family 4 subfamily N member 2
Transcript:
RefSeq XM_372500.1 (NON-CURRENT)
Match location:
Position 655 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000194310)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGTCATTCTTTGTCGCATA
Hairpin Sequence:
5'-CCGG-GCAGTCATTCTTTGTCGCATA-CTCGAG-TATGCGACAAAGAATGACTGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGTCATTCTTTGTCGCATACTCGAGTATGCGACAAAGAATGACTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGTCATTCTTTGTCGCATACTCGAGTATGCGACAAAGAATGACTGC-3'

Other clones with same target sequence:

(none)