Construct: shRNA TRCN0000194352

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_013721.2-627s1c1
DNA Barcode:
CGTTGCCTTCACACAGGTTAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Rpl7a (27176)
Gene Description:
ribosomal protein L7A
Transcript:
RefSeq NM_013721.2 (NON-CURRENT)
Match location:
Position 627 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000194352)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGTTGCCTTCACACAGGTTAA
Hairpin Sequence:
5'-CCGG-CGTTGCCTTCACACAGGTTAA-CTCGAG-TTAACCTGTGTGAAGGCAACG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGTTGCCTTCACACAGGTTAACTCGAGTTAACCTGTGTGAAGGCAACGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGTTGCCTTCACACAGGTTAACTCGAGTTAACCTGTGTGAAGGCAACG-3'

Other clones with same target sequence:

(none)