Construct: shRNA TRCN0000194442

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_018733.1-634s1c1
DNA Barcode:
GATCAGCGAAAGACGATCAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Scn1a (20265)
Gene Description:
sodium channel, voltage-gated, type I, alpha
Transcript:
RefSeq NM_018733.1 (NON-CURRENT)
Match location:
Position 634 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000194442)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GATCAGCGAAAGACGATCAAA
Hairpin Sequence:
5'-CCGG-GATCAGCGAAAGACGATCAAA-CTCGAG-TTTGATCGTCTTTCGCTGATC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGATCAGCGAAAGACGATCAAACTCGAGTTTGATCGTCTTTCGCTGATCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGATCAGCGAAAGACGATCAAACTCGAGTTTGATCGTCTTTCGCTGATC-3'

Other clones with same target sequence:

(none)