Construct: shRNA TRCN0000194475

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_146078.2-908s1c1
DNA Barcode:
GCAACTACAGTTGATCGAGAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ubr2 (224826)
Gene Description:
ubiquitin protein ligase E3 component n-recognin 2
Transcript:
RefSeq NM_146078.2 (NON-CURRENT)
Match location:
Position 908 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000194475)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAACTACAGTTGATCGAGAT
Hairpin Sequence:
5'-CCGG-GCAACTACAGTTGATCGAGAT-CTCGAG-ATCTCGATCAACTGTAGTTGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAACTACAGTTGATCGAGATCTCGAGATCTCGATCAACTGTAGTTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAACTACAGTTGATCGAGATCTCGAGATCTCGATCAACTGTAGTTGC-3'

Other clones with same target sequence:

(none)