Construct: shRNA TRCN0000194492

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_180588.1-979s1c1
DNA Barcode:
GCACAGGGAGACATTCACTAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Reep4 (72549)
Gene Description:
receptor accessory protein 4
Transcript:
RefSeq NM_180588.1 (NON-CURRENT)
Match location:
Position 979 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000194492)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCACAGGGAGACATTCACTAT
Hairpin Sequence:
5'-CCGG-GCACAGGGAGACATTCACTAT-CTCGAG-ATAGTGAATGTCTCCCTGTGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCACAGGGAGACATTCACTATCTCGAGATAGTGAATGTCTCCCTGTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCACAGGGAGACATTCACTATCTCGAGATAGTGAATGTCTCCCTGTGC-3'

Other clones with same target sequence:

(none)