Construct: shRNA TRCN0000194505

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_026557.2-983s1c1
DNA Barcode:
GAATACCAGAACGTGACTGTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Rchy1 (68098)
Gene Description:
ring finger and CHY zinc finger domain containing 1
Transcript:
RefSeq NM_026557.2 (NON-CURRENT)
Match location:
Position 983 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000194505)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAATACCAGAACGTGACTGTT
Hairpin Sequence:
5'-CCGG-GAATACCAGAACGTGACTGTT-CTCGAG-AACAGTCACGTTCTGGTATTC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAATACCAGAACGTGACTGTTCTCGAGAACAGTCACGTTCTGGTATTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAATACCAGAACGTGACTGTTCTCGAGAACAGTCACGTTCTGGTATTC-3'

Other clones with same target sequence:

(none)