Construct: shRNA TRCN0000194517
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_028049.1-1717s1c1
- DNA Barcode:
- GCCTGGTCTATAGAGTGAGTT
Original Target:
- Taxon:
- Mus musculus (mouse)
- Gene:
- Fbxo22 (71999)
- Gene Description:
- F-box protein 22
- Transcript:
- RefSeq NM_028049.1 (NON-CURRENT)
- Match location:
- Position 1717 (3UTR)
Vector Information:
- Vector Backbone:
- pLKO.1
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000194517)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
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Sequence Information
- Target Sequence:
- GCCTGGTCTATAGAGTGAGTT
- Hairpin Sequence:
- 5'-CCGG-GCCTGGTCTATAGAGTGAGTT-CTCGAG-AACTCACTCTATAGACCAGGC-TTTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGGCCTGGTCTATAGAGTGAGTTCTCGAGAACTCACTCTATAGACCAGGCTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAAGCCTGGTCTATAGAGTGAGTTCTCGAGAACTCACTCTATAGACCAGGC-3'