Construct: shRNA TRCN0000194521

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_178924.2-162s1c1
DNA Barcode:
GACAGCAGAGTGTATCTTCTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Upk1b (22268)
Gene Description:
uroplakin 1B
Transcript:
RefSeq NM_178924.2 (NON-CURRENT)
Match location:
Position 162 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000194521)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GACAGCAGAGTGTATCTTCTT
Hairpin Sequence:
5'-CCGG-GACAGCAGAGTGTATCTTCTT-CTCGAG-AAGAAGATACACTCTGCTGTC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGACAGCAGAGTGTATCTTCTTCTCGAGAAGAAGATACACTCTGCTGTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGACAGCAGAGTGTATCTTCTTCTCGAGAAGAAGATACACTCTGCTGTC-3'

Other clones with same target sequence:

(none)