Construct: shRNA TRCN0000194566

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_175475.2-919s1c1
DNA Barcode:
CCACCTCTTTGAGGTATACCA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Cyp26b1 (232174)
Gene Description:
cytochrome P450, family 26, subfamily b, polypeptide 1
Transcript:
RefSeq NM_175475.2 (NON-CURRENT)
Match location:
Position 919 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000194566)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCACCTCTTTGAGGTATACCA
Hairpin Sequence:
5'-CCGG-CCACCTCTTTGAGGTATACCA-CTCGAG-TGGTATACCTCAAAGAGGTGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCACCTCTTTGAGGTATACCACTCGAGTGGTATACCTCAAAGAGGTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCACCTCTTTGAGGTATACCACTCGAGTGGTATACCTCAAAGAGGTGG-3'

Other clones with same target sequence:

(none)