Construct: shRNA TRCN0000194618

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011716.1-392s1c1
DNA Barcode:
GCCAACGATGCAGATGAAGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Wfs1 (22393)
Gene Description:
wolframin ER transmembrane glycoprotein
Transcript:
RefSeq NM_011716.1 (NON-CURRENT)
Match location:
Position 392 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000194618)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCAACGATGCAGATGAAGAA
Hairpin Sequence:
5'-CCGG-GCCAACGATGCAGATGAAGAA-CTCGAG-TTCTTCATCTGCATCGTTGGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCAACGATGCAGATGAAGAACTCGAGTTCTTCATCTGCATCGTTGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCAACGATGCAGATGAAGAACTCGAGTTCTTCATCTGCATCGTTGGC-3'

Other clones with same target sequence:

(none)